Oberon, King of the Fairies, is arguing with his queen Tytania over a young changeling boy. So Oberon sends Puck to find a magical flower whose juice, sprinkled on Tytania’s eyelids, will cause her to fall in love with the first creature she sees.
Meanwhile, lovers Lysander and Hermia have eloped to the forest. They are running away from Demetrius, who is in love with Hermia, and who in turn is loved by Helena! Oberon has witnessed their troubles and orders Puck to use the magic juice on Demetrius too, so that he will fall in love with Helena.
Six craftsmen arrive in the forest to discuss a play they hope to perform at the wedding of Theseus and Hippolyta. They argue over casting, but finally Bottom and Flute agree to play the title roles, Pyramus and Thisbe.
Lysander and Hermia go to sleep. Puck mistakes Lysander for Demetrius and sprinkles the juice of the magic flower on Lysander’s eyes. Demetrius arrives with Helena soon after. Helena sees Lysander and wakes him. Under the spell, Lysander is smitten with her. She runs off, believing he is mocking her, and Hermia awakes alone.
Nearby, the fairies prepare Tytania for sleep. Oberon slips in to sprinkle the magic juice on her eyes…